NC Membrane, 0.22 μm
NC Membrane, 0.22 μm is a nitrocellulose membrane widely used as a blotting membrane for proteins and nucleic acids.
Product overview
NC Membrane, 0.22 μm is a nitrocellulose membrane widely used as a blotting membrane for proteins and nucleic acids. The membrane is hydrophilic and does not require activation with methanol or ethanol; it only needs brief wetting with water prior to protein transfer. Its uniform pore structure allows efficient binding of biomolecules and is especially suitable for immunoblotting (Western blot) to transfer proteins from various gel matrices onto the membrane. The 0.22 μm pore size offers high adsorption efficiency for proteins with molecular weights below 20 kDa.
Features and applications
- No methanol activation required.
- Protein binding capacity > 115 μg/cm².
- Low fluorescence background.
- Transfer and detection of low molecular weight proteins (< 20 kDa) in Western blot workflows.
- Highly suitable for chemiluminescent, colorimetric, and fluorescent detection systems.
Components
| Components | Specification |
|---|---|
| NC Membrane, 0.22 μm | 90 mm×70 mm×20 sheets |
Performance data
PVDF vs NC Membrane Selection Guide
| Product | NC Membrane | PVDF Membrane |
|---|---|---|
| Pore Size | 0.22 μm, 0.45 μm | 0.22 μm, 0.45 μm |
| Fluorescence Background | Very low | Higher |
| Methanol Pre-wetting | No | Yes |
| Stripping and Reprobing | Not recommended | Recommended |
| Protein Binding Capacity | >115 μg/cm2 | >200 μg/cm2 |
| Digestion or Mass Spectrometry Analysis | No | No |
| Detection Methods | Fluorescence; Chemiluminescence; Chemifluorescence; Colorimetric | Chemiluminescence; Isotope/Radioactive; Chemifluorescence; Colorimetric |
| Recommended Applications | Western blotting; Structural analysis; Amino acid analysis; Dot/Slot blot | Western blotting; Structural analysis; Amino acid analysis; Glycoprotein analysis; Lipopolysaccharide analysis; Dot/Slot blot |
| For proteins with molecular weights greater than 20 kDa, a 0.45 μm membrane is recommended. For proteins with molecular weights less than 20 kDa, a 0.22 μm membrane is generally recommended for more efficient capture. | ||
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at 16-30°C. |
| Shipping | Ambient transport. |
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