5× Rapid Blocking Buffer

5× Rapid Blocking Buffer is developed for rapid and efficient blocking of transfer membranes.

5–15 min

Membrane blocking

Higher S/N

vs non-fat milk or BSA

Phospho antibodies

Biotin systems compatible

Product overview

5× Rapid Blocking Buffer is developed for rapid and efficient blocking of transfer membranes. This protein-free formulation blocks only the membrane and does not mask antigenic epitopes on sample proteins, thereby improving the efficiency of specific antibody binding. It helps reduce non-specific antibody binding and background interference, delivering clear target signals with a high signal-to-noise ratio. It does not contain endogenous phosphoproteins or biotin, avoiding interference with phospho-specific antibody binding and biotin-based detection workflows while supporting accurate results. This product is broadly applicable to phospho-antibody assays and biotin detection systems. Efficient and convenient membrane blocking can be completed within 5-15 min. This product is supplied as a 5× concentrate and should be diluted to a 1× working solution before use.

Features and applications

Features

Protein-free formulation that does not mask protein antigenic epitopes and improves the efficiency of specific-antibody binding.
Helps reduce non-specific antibody binding and background interference for clear target signals and a high signal-to-noise ratio.
Free of endogenous phosphoproteins and biotin to avoid interference with phospho-specific antibody and biotin-based detection.
Fast blocking: membrane blocking completed within 5-15 min.
Supplied as 5× concentrate; simply dilute to prepare 1× working solution before use.

Applications