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Product Catalog
E. coli Residual DNA Detection Kit (qPCR), BP-QN24, provides quantitative detection of E. coli residual DNA in intermediates, semi-finished products, and finished biological or pharmaceutical products. The fluorescent probe qPCR workflow includes an internal standard and calibrators for residual host-cell DNA testing.
E.coli Residual DNA Detection Kit (qPCR), BP-QN24
USD
$0.00
BP-QN24-100
Features
- qPCR probe-based assay targeting conserved E.coli genomic sequences.
- Includes an exogenous internal standard for extraction and amplification monitoring.
- UDG enzyme is included to help prevent carryover contamination from amplification products.
- Five E.coli calibrators are supplied from 30 fg/uL to 300 pg/uL.
- COA reports conforming precision, accuracy, amplification efficiency, linear correlation, and internal-standard Ct results.
Application
- Quantification of E.coli residual DNA in biopharmaceutical process samples.
- Testing of intermediates, semi-finished products, and finished products.
- qPCR workflows using supported real-time PCR systems listed in the manual.
- Sample ERC workflows when paired with a suitable nucleic acid extraction or purification kit.
Components
| Component | Specification |
|---|---|
| E.coli qPCR MIX | 1.0 mL x 2 tubes |
| E.coli Internal Standard | 1.0 mL x 1 tube |
| E.coli Negative Control | 1.0 mL x 2 tubes |
| E.coli Calibrator ST1 | 0.5 mL x 1 tube; 30 fg/uL |
| E.coli Calibrator ST2 | 0.5 mL x 1 tube; 300 fg/uL |
| E.coli Calibrator ST3 | 0.5 mL x 1 tube; 3 pg/uL |
| E.coli Calibrator ST4 | 0.5 mL x 1 tube; 30 pg/uL |
| E.coli Calibrator ST5 | 0.5 mL x 1 tube; 300 pg/uL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | <= -20 C, protected from light. Shelf life: 24 months. Avoid more than 10 freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
