NC Membrane, 0.45 μm
NC Membrane, namely nitrocellulose membrane, is one of the most commonly used blotting membranes for protein and nucleic acid blotting.
Product overview
NC Membrane, namely nitrocellulose membrane, is one of the most commonly used blotting membranes for protein and nucleic acid blotting. NC membrane is hydrophilic and requires no activation with methanol or ethanol; it only needs simple wetting with water before transfer. It features a uniform pore structure and can efficiently bind biomolecules. It is mainly applied to Western blot, enabling the transfer of proteins from various gel matrices onto the membrane. This 0.45 μm NC membrane exhibits higher adsorption efficiency for proteins with molecular weights greater than 20 kDa.
Features and applications
- No methanol activation required.
- Protein binding capacity > 115 μg/cm².
- Low fluorescence background.
- Transfer and detection of proteins > 20 kDa in Western blot workflows.
- Highly suitable for chemiluminescent, colorimetric, and fluorescent detection systems.
Components
| Components | Specification |
|---|---|
| NC Membrane, 0.45 μm | 90 mm×70 mm×20 sheets |
Performance data
PVDF vs NC Membrane Selection Guide
| Product | NC Membrane | PVDF Membrane |
|---|---|---|
| Pore Size | 0.22 μm, 0.45 μm | 0.22 μm, 0.45 μm |
| Fluorescence Background | Very low | Higher |
| Methanol Pre-wetting | No | Yes |
| Stripping and Reprobing | Not recommended | Recommended |
| Protein Binding Capacity | >115 μg/cm2 | >200 μg/cm2 |
| Digestion or Mass Spectrometry Analysis | No | No |
| Detection Methods | Fluorescence; Chemiluminescence; Chemifluorescence; Colorimetric | Chemiluminescence; Isotope/Radioactive; Chemifluorescence; Colorimetric |
| Recommended Applications | Western blotting; Structural analysis; Amino acid analysis; Dot/Slot blot | Western blotting; Structural analysis; Amino acid analysis; Glycoprotein analysis; Lipopolysaccharide analysis; Dot/Slot blot |
| For proteins with molecular weights greater than 20 kDa, a 0.45 μm membrane is recommended. For proteins with molecular weights less than 20 kDa, a 0.22 μm membrane is generally recommended for more efficient capture. | ||
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at 16-30°C. |
| Shipping | Ambient transport. |
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