PVDF Membrane, 0.45 μm
PVDF Membrane, 0.45 μm is a polyvinylidene fluoride (PVDF) membrane widely used as a solid support for protein blotting.
Product overview
PVDF Membrane, 0.45 μm is a polyvinylidene fluoride (PVDF) membrane widely used as a solid support for protein blotting. PVDF membranes are hydrophobic and available in different pore sizes; as the pore size decreases, their binding capacity for low-molecular-weight proteins becomes stronger. This product has a pore size of 0.45 μm and is suitable for most immunoblotting applications, particularly for proteins with molecular weights greater than 20 kDa. Before use, the membrane should be pretreated, typically by soaking in methanol, to activate the membrane surface and improve protein binding. Because of its high mechanical strength, PVDF Membrane, 0.45 μm is an ideal solid-support material for protein blotting. It is most commonly used for protein transfer from gels in Western blotting and may also be used for adsorption analysis, amino acid analysis, N-terminal protein sequencing, dot blot and slot blot detection, glycoprotein staining, and lipopolysaccharide analysis.
Features and applications
- Protein binding capacity > 200 μg/cm².
- Relatively high fluorescence background.
- Transfer and detection of proteins > 20 kDa in Western blot workflows.
- Highly suitable for chemiluminescent and colorimetric detection systems.
- Excellent mechanical strength, making it ideal for stripping and reprobing protocols.
Components
| Components | Specification |
|---|---|
| PVDF Membrane, 0.45 μm | 80 mm×60 mm×20 sheets |
Performance data
PVDF vs NC Membrane Selection Guide
| Product | NC Membrane | PVDF Membrane |
|---|---|---|
| Pore Size | 0.22 μm, 0.45 μm | 0.22 μm, 0.45 μm |
| Fluorescence Background | Very low | Higher |
| Methanol Pre-wetting | No | Yes |
| Stripping and Reprobing | Not recommended | Recommended |
| Protein Binding Capacity | >115 μg/cm2 | >200 μg/cm2 |
| Digestion or Mass Spectrometry Analysis | No | No |
| Detection Methods | Fluorescence; Chemiluminescence; Chemifluorescence; Colorimetric | Chemiluminescence; Isotope/Radioactive; Chemifluorescence; Colorimetric |
| Recommended Applications | Western blotting; Structural analysis; Amino acid analysis; Dot/Slot blot | Western blotting; Structural analysis; Amino acid analysis; Glycoprotein analysis; Lipopolysaccharide analysis; Dot/Slot blot |
| For proteins with molecular weights greater than 20 kDa, a 0.45 μm membrane is recommended. For proteins with molecular weights less than 20 kDa, a 0.22 μm membrane is generally recommended for more efficient capture. | ||
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at 16-30°C. |
| Shipping | Ambient transport. |
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