Vaccinia Capping Enzyme GMP-grade
Enzyme for Cap0 mRNA capping workflows.
Product overview
Mature mRNA in eukaryotes carries a unique 5' cap structure (m7GpppN), known as the methylguanosine cap, which is essential for translation initiation, protection from RNase degradation, and enhancement of mRNA stability during splicing and nuclear export. Vaccinia Capping Enzyme GMP-grade (VCE), developed in-house by Biori Biotech, integrates the three enzymatic activities required for capping: RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase. In the presence of S-adenosylmethionine (SAM) as a methyl donor and guanosine triphosphate (GTP), VCE directly and efficiently adds an m7G cap to the 5' end of mRNA in the correct orientation, producing Cap0-capped RNA in a single reaction. Cap1 RNA can be obtained by using VCE in combination with mRNA Cap 2'-O-Methyltransferase GMP-grade (GMP-BP-E06).
Available versions
| Cat. no. | Product name | Formulation |
|---|---|---|
| GMP-BP-E05-1K | Vaccinia Capping Enzyme GMP-grade | |
| GMP-BP-E05-10K | Vaccinia Capping Enzyme GMP-grade | |
| GMP-BP-E05-100K | Vaccinia Capping Enzyme GMP-grade |
Features and applications
- Integrates RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase activities; activity: 10 U/μL.
- Unit definition: one unit (U) is defined as the amount of enzyme required to incorporate 10 pmol of GTP into RNA in 1 hour at 37°C.
- Quality control: protein purity ≥95%; free of exogenous DNase, RNase, exonuclease, and endonuclease activity; residual host-cell DNA ≤100 pg/mg; residual host-cell protein <50 ppm; bacterial endotoxin <5 EU/mL.
- Cap 0 capping of mRNA for in vitro or in vivo translation.
- For Cap 1 structure, use in combination with mRNA Cap 2'-O-Methyltransferase GMP-grade (GMP-BP-E06).
Components
| Component | GMP-BP-E05-1K | GMP-BP-E05-10K | GMP-BP-E05-100K |
|---|---|---|---|
| 1 KU | 10 KU | 100 KU | |
| Vaccinia Capping Enzyme GMP-grade (10 U/μL) | 0.1 mL | 1 mL | 10 mL |
| 10× Capping Buffer | 0.2 mL | 2 mL | 20 mL |
| GTP Solution (10 mM) | 0.1 mL | 1 mL | 10 mL |
| SAM (32 mM) | 20 μL | 0.2 mL | 2 mL |
Performance data
1 Functional test
Description: The VCE is mainly used for adding caps to post-transcriptional samples. The cap addition rate is determined by liquid chromatography-mass spectrometry to reflect the cap-adding ability of the VCE enzyme to mRNA. Study the cap-forming ability of different doses of VCE.
Conclusion: Low-dose VCE can achieve a 95% capping rate, while medium-dose and high-dose can both achieve a 100% capping rate.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Downloads
Need this product lyophilized, in bulk, or in a custom format?
The feasibility assessment is free of charge. Send the assay brief and target format.
