Western Blot Reagents from Extraction to Detection
RIPA buffers and inhibitor cocktails, BCA and Bradford assays, PAGE gel kits and markers, PVDF and NC membranes, transfer, blocking and TBST buffers, DAB and ECL substrates.
Pick the reagent by workflow step
Start from what you need to do. Each row names the product we would pick first and the alternatives in the same group; the full list is below.
| Use case | Recommended product | Why this one | Alternatives |
|---|---|---|---|
| Transfer & Blocking | 10× Fast Transfer Buffer BR4D421 Data | 10× Fast Transfer Buffer is a high-efficiency, safe, and non-toxic transfer buffer developed for rapid wet transfer in Western blot workflows. | BR4D451, BR4D441, BR4D414, BR4D413, BR4D412, BR4D411 |
| Protein Electrophoresis | 10× Tris-Glycine-SDS Running Buffer BR4C361 | 10× Tris-Glycine-SDS Running Buffer is commonly used as the electrophoresis buffer for protein SDS-PAGE. | BR4C303, BR4C351, BR4C333, BR4C334, BR4C335, BR4C331, BR4C332 |
| Protein Quantification | BCA Protein Assay Kit BR4C201 | Colorimetric BCA protein assay for microplates: 37°C for 30 min, read at 562 nm, BSA standard curve 0–300 μg/mL (R² ≥ 0.99). | BR4C202 |
| Protein Extraction | Cell Total Protein Extraction BR4C131 | Cell Total Protein Extraction Kit is designed for the extraction of total proteins from various animal cells, fungi, or bacterial samples. | BR4C122, BR4C123, BR4C121, BR4C102, BR4C101, BR4C103, BR4C104 |
| Detection & Imaging | DAB Peroxidase Substrate Kit BR4D601 Data | DAB Peroxidase Substrate Kit is a colorimetric substrate kit developed for horseradish peroxidase (HRP) detection. | BR4D602 |
27 products
10× Fast Transfer Buffer
10× Fast Transfer Buffer is a high-efficiency, safe, and non-toxic transfer buffer developed for rapid wet transfer in Western blot workflows.
10× TBST Buffer
10× TBST Buffer is an isotonic buffered saline solution primarily used in Western Blot assays to wash away antibodies and other reagents bound non-specifically to membranes.
5× Rapid Blocking Buffer
5× Rapid Blocking Buffer is developed for rapid and efficient blocking of transfer membranes.
NC Membrane, 0.22 μm
NC Membrane, 0.22 μm is a nitrocellulose membrane widely used as a blotting membrane for proteins and nucleic acids.
NC Membrane, 0.45 μm
NC Membrane, namely nitrocellulose membrane, is one of the most commonly used blotting membranes for protein and nucleic acid blotting.
PVDF Membrane, 0.22 μm
PVDF Membrane, 0.22 μm is a polyvinylidene fluoride (PVDF) membrane widely used as a solid support for protein blotting.
PVDF Membrane, 0.45 μm
PVDF Membrane, 0.45 μm is a polyvinylidene fluoride (PVDF) membrane widely used as a solid support for protein blotting.
10× Tris-Glycine-SDS Running Buffer
10× Tris-Glycine-SDS Running Buffer is commonly used as the electrophoresis buffer for protein SDS-PAGE.
180 kDa Prestained Protein Marker
180 kDa Prestained Protein Marker consists of 10 prestained proteins in three colors with molecular weights in the range of 13-180 kDa.
5× SDS-PAGE Protein Loading Buffer
5× SDS-PAGE Protein Loading Buffer is a 5× concentrated protein sample loading buffer.
One-Step PAGE Gel Fast Preparation Kit (10%)
One-Step PAGE Gel Fast Preparation Kit (10%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.
One-Step PAGE Gel Fast Preparation Kit (12.5%)
One-Step PAGE Gel Fast Preparation Kit (12.5%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.
One-Step PAGE Gel Fast Preparation Kit (15%)
One-Step PAGE Gel Fast Preparation Kit (15%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.
One-Step PAGE Gel Fast Preparation Kit (6%)
One-Step PAGE Gel Fast Preparation Kit (6%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.
One-Step PAGE Gel Fast Preparation Kit (7.5%)
One-Step PAGE Gel Fast Preparation Kit (7.5%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.
BCA Protein Assay Kit
Colorimetric BCA protein assay for microplates: 37°C for 30 min, read at 562 nm, BSA standard curve 0–300 μg/mL (R² ≥ 0.99).
Bradford Protein Assay Kit
Ready-to-use Coomassie G-250 protein assay: 3-5 min at room temperature, read at 595 nm, optimal range 100-500 μg/mL.
Cell Total Protein Extraction
Cell Total Protein Extraction Kit is designed for the extraction of total proteins from various animal cells, fungi, or bacterial samples.
Phosphatase Inhibitor Cocktail (100×)
Phosphatase Inhibitor Cocktail (100×) contains sodium fluoride, β-glycerophosphate, and sodium orthovanadate as phosphatase inhibitors for cell or tissue extracts.
Protease and Phosphatase Inhibitor Cocktail, EDTA-Free (100×)
Protease and Phosphatase Inhibitor Cocktail, EDTA-Free (100×) combines six protease inhibitors and three phosphatase inhibitors to protect proteins in cell or tissue extracts from degradation and dephosphorylation.
Protease Inhibitor Cocktail, EDTA-Free (100×)
Protease Inhibitor Cocktail, EDTA-Free (100×) contains AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, and Pepstatin A to prevent protein degradation in cell or tissue extracts.
RIPA Lysis Buffer (Medium)
Medium-lysis RIPA buffer with excellent cytoplasmic and good membrane and nuclear protein extraction for WB and IP.
RIPA Lysis Buffer (Strong)
Strong-lysis RIPA buffer with excellent membrane, cytoplasmic and nuclear protein extraction for WB and IP.
RIPA Lysis Buffer (Weak)
Weak-lysis RIPA buffer with excellent cytoplasmic protein extraction for WB, IP and Co-IP.
RIPA Lysis Buffer Set
Strong, Medium and Weak RIPA lysis buffers (50 mL each) to match membrane, cytoplasmic and nuclear protein extraction.
DAB Peroxidase Substrate Kit
DAB Peroxidase Substrate Kit is a colorimetric substrate kit developed for horseradish peroxidase (HRP) detection.
ECL Chemiluminescent Substrate Kit
ECL Chemiluminescent Substrate Kit is a chemiluminescent detection kit that contains all reagents required for the detection of horseradish peroxidase (HRP).
Documents
Manuals, COA and SDS are linked on each product row and on the product page.
Custom and OEM
Bulk fills, custom formulations and pilot evaluation are quoted per project.
Talk to an expert →Frequently asked questions
Which products are in the Western Blot (WB) category?
What documents are provided?
Can I order bulk packs or a custom formulation?
Need help choosing, or a custom format?
Send your workflow and requirements. We reply with a proposal and evaluation samples.
